Figure 4. Knockdown of α-synuclein in the primary cultured hippocampal neuron in vitro. (A and B) The interference efficiency of α-synuclein siRNA was confirmed by qRT-PCR (A) and Western blotting (B). (C) Immunofluorescence staining of control or α-synuclein siRNA in cultured hippocampal neuron. Scale bar, 30 μm for the low-power field in the left and 15 μm for the high-power field in the right. (D and E) Effects of α-synuclein siRNA on the glutamate receptors and synaptic proteins expressions in cultured hippocampal neuron. (F) Effects of Rb1 on the glutamate receptors and synaptic proteins expressions in cultured hippocampal neuron. n= 10 for (C), n = 7 per group for (A), n = 6 for (B) and (D–G). Scale bar, 80 μm. Results are expressed as the mean ± SEM of three experiments. **p < 0.01, *p < 0.05 vs. control group. Statistical significance was determined by Student's t-test for (C), and one-way ANOVA and Bonferroni tests as post hoc comparisons for (A, B) and (D–G).