Figure 10. Low-dose Vitamin C alone increases both mitochondrial metabolism and glycolysis: Bar graphs. The metabolic profile of MCF7 cell monolayers pre-treated with 250 μM Vitamin C alone for 3 days was assessed using the Seahorse XFe96 analyzer. Note that treatment with 250 μM Vitamin C significantly increased basal respiration, ATP production and maximal respiration. Also, note that treatment with 250 μM Vitamin C significantly increased glycolysis and glycolytic reserves, while decreasing glycolytic reserve capacity. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.