Research Paper Volume 11, Issue 7 pp 2151—2176

Pyridoxine 5′-phosphate oxidase is correlated with human breast invasive ductal carcinoma development

Figure 10. Effect of miR-216b-5p on PNPO expression in breast cancer cells. (A) Identification of the sequence of binding sites between PNPO mRNA 3’-UTR and miR-216b-5p. PNPO-wt, wild-type PNPO; PNPO-mut, mutated PNPO. (B) Detection of luciferase activity by the dual-luciferase reporter assay in HEK-293T cells. (C) Detection of miR-216b-5p by qRT-PCR in MCF-7 cells after miR-216b mimics treatment. (D) Effect of miR-216b mimics on PNPO expression detected by Western blot (left) and semi-quantification (right) in MCF-7 cells. (E) Detection of miR-216b-5p by qRT-PCR in MCF-7 cells after miR-216b inhibitors treatment. (F) Effect of miR-216b inhibitors on PNPO expression detected by Western blot (left) and semi-quantification (right) in MCF-7 cells. (G) Detection of miR-216b-5p by qRT-PCR in MDA-MB-231 cells after miR-216b mimics treatment. (H) Effect of miR-216b mimics on PNPO expression detected by Western blot (left) and semi-quantification (right) in MDA-MB-231 cells. (I) Detection of miR-216b-5p by qRT-PCR in MDA-MB-231 cells after miR-216b inhibitors treatment. (J) Effect of miR-216b inhibitors on PNPO expression detected by Western blot (left) and semi-quantification (right) in MDA-MB-231. miR-NC, negative control of miRNA. n = 3; * P < 0.05.