Figure 6. The effect of PNPO on breast cell migration and invasion. (A) Effect of PNPO knockdown by PNPO-shRNA on cell migration in MCF-12A, MCF-7, and MDA-MB-231 cells assessed by the wound-healing assay (Original magnification, x500). (B) Histograms show the quantitative analyses of wound distance at 0 and 48 h post-scratch (n = 3). (C) Effect of PNPO knockdown by PNPO-shRNA on cell invasion in MCF-12A, MCF-7, and MDA-MB-231 cells detected by the Transwell assay (Original magnification, x200). (D) Histograms show the number of invading cells (n = 3). NC, negative control of shRNA; shPNPO, PNPO-shRNA. * P < 0.05.