Research Paper Volume 11, Issue 7 pp 2151—2176
Pyridoxine 5′-phosphate oxidase is correlated with human breast invasive ductal carcinoma development
Figure 8. The effect of PNPO on breast cell apoptosis. (A–C) Apoptotic cells were detected by flow cytometry after PNPO knockdown in MCF-12A (A), MCF-7 (B), and MDA-MB-231 (C) cells. (D–F) Expression of caspase-3 and PNPO protein after PNPO knockdown in MCF-12A (D), MCF-7 (E), and MDA-MB-231 (F) cells detection by Western blot. (G–I) Expression of Bcl-2 and Bax protein after PNPO knockdown in MCF-12A (G), MCF-7 (H), and MDA-MB-231 (I) cells detection by Western blot. Histograms show the quantitative analyses. NC, negative control of shRNA; shPNPO, PNPO-shRNA. n =3; * P < 0.05.