Research Paper Volume 11, Issue 8 pp 2217—2240

Resveratrol prevents sarcopenic obesity by reversing mitochondrial dysfunction and oxidative stress via the PKA/LKB1/AMPK pathway

Figure 10. RSV improves protein metabolism via the PKA/LKB1/AMPK signaling pathway. (A) Representative images of the western blotting results for p-mTOR, mTOR, p-S6K, S6K, FoxO3a, atrogin-1, MuRF1 and MHC in L6 myotubes; GAPDH was used as a loading control. (B and C) The bar graphs show quantification of the indicated proteins. Data are expressed as the mean ± SD. aP < 0.05 vs. the control group; bP < 0.05 vs. the PA-treated group; cP < 0.05 vs. the RSV (25 μM) and PA (0.75 mM) cotreated group with control siRNA transfection. (D) Representative images of the western blotting results for p-mTOR, mTOR, p-S6K, S6K, FoxO3a, atrogin-1 and MuRF1 in the GAS muscles; GAPDH was used as a loading control. (E) The bar charts show the relative protein levels. Data are expressed as the mean ± SD. aP < 0.05 vs. the O group; bP < 0.05 vs. the HO group.