Research Paper Volume 11, Issue 8 pp 2217—2240

Resveratrol prevents sarcopenic obesity by reversing mitochondrial dysfunction and oxidative stress via the PKA/LKB1/AMPK pathway

Figure 3. Effects of RSV on mitochondrial morphology and related protein expression in the GAS muscles. (A and B) Representative TEM images of the IFM and SSM at an original magnification of 25,000×. The short arrows indicate damaged mitochondria; the long arrows indicate lipid droplets. (C) Representative images of the western blotting results for PGC-1α, TFAM, mfn2 and drp1 in whole muscle lysates; GAPDH was used as a loading control. (D) The bar graphs show quantification of the indicated proteins. (E) Representative images of the western blotting results for TFAM, mfn2 and drp1 in the mitochondrial subfractions; VDAC1 was used as a loading control. (F) The bar graphs show quantification of the indicated proteins. (G) Relative mtDNA content. Data are expressed as the mean ± SD. aP < 0.05 vs. the O group; bP < 0.05 vs. the HO group.