Research Paper Volume 11, Issue 8 pp 2217—2240

Figure 7. Effects of RSV on mitochondrial morphology, mtDNA content and related protein expression in L6 myotubes. (A) Representative images for visualization of the mitochondrial morphology in vitro. Scale bar, 10 μm. (B) The mitochondrial mass was quantified by calculating the fluorescent intensity of MitoTracker Red. (C) Relative mtDNA content. (D) The levels of PGC-1α, TFAM, mfn2 and drp1 in the whole muscle lysates were measured by western blotting; GAPDH was used as a loading control. (E) The bar charts show quantification of the indicated proteins. (F) Representative images of the western blotting results for TFAM, mfn2 and drp1 in the mitochondrial subfractions; VDAC1 was used as a loading control. (G) The bar charts show quantification of the indicated proteins. Data are expressed as the mean ± SD. ^aP < 0.05 vs. the control group; ^bP < 0.05 vs. the PA-treated group; ^cP < 0.05 vs. the RSV (25 μM) and PA (0.75 mM) cotreated group with control siRNA transfection.