Research Paper Volume 11, Issue 8 pp 2241—2252

Exposure to aristolochic acid I compromises the maturational competency of porcine oocytes via oxidative stress-induced DNA damage

Figure 3. Effects of AAI exposure on the acetylation level of α-tubulin in porcine oocytes. (A) Representative images of acetylated α-tubulin (Ac-Tub) in control and AAI-exposed oocytes. Oocytes were immunostained with anti-acetyl-α-tubulin (Lys-40) antibody to assess the acetylation level of α-tubulin. Scale bar, 10 μm. (B) Quantitative measurement of the fluorescence intensity of acetylated α-tubulin in control and AAI-exposed oocytes. Data were presented as mean percentage (mean ± SEM) of at least three independent experiments. ***P < 0.001. (C) The acetylation levels of α-tubulin in control and AAI-exposed oocytes were examined by western blotting. The blots were probed with anti-acetyl-α-tubulin (Lys-40) antibody and anti-Gapdh antibody, respectively.