Research Paper Volume 11, Issue 8 pp 2281—2294

Non-genomic mechanisms mediate androgen-induced PSD95 expression

Figure 1. T-BSA rapidly increased PSD95 expression through a transcription-independent mechanism. (A and B) Time-dependent effects of T-BSA (0 min, 5 min, 15 min, 30 min and 60min) on PSD95 protein levels (n=5). (C and D) Dose-dependent effects of T-BSA (DMSO, 5 nM, 10 nM, 15 nM and 20 nM) on PSD95 protein levels (n=5). (E and F) FITC signals on the HT22 cell plasma membrane (n=5, scale bars = 50 μm). (G and H) Western blotting for PSD95 expression induced by T-BSA in HT22 cells pre-treated with 10 μM Act D or 200 μM CHX for 2 h (n=5). (I and J) Immunofluorescence staining for PSD95 induced by T-BSA in HT22 cells pre-treated with Act D or CHX (n=4, scale bars = 20 μm). (K and L) RT-PCR for PSD95 mRNA level induced by T-BSA (n=5). (n.s.: non-significant; * P < 0.05; ** P < 0.01).