Research Paper Volume 11, Issue 8 pp 2281—2294

Non-genomic mechanisms mediate androgen-induced PSD95 expression

Figure 2. T-BSA increased PSD95 expression via ZIP9 rather than membrane-localized AR. (A and B) Western blotting for PSD95 expression induced by T-BSA in HT22 cells pre-treated with flutamide (F, 100 μM) for 1 h (n=5). (C and D) Immunofluorescence staining for PSD95 induced by T-BSA in HT22 cells pretreated with flutamide (n=4, scale bar = 20 μm). (E and F) Western blotting for the knockdown efficiency of ZIP9 protein in HT22 cells transfected with nc-shRNA or ZIP9-shRNA (n=5). (G and H) Western blotting for PSD95 expression induced by T-BSA in HT22 cells pre-treated with nc-shRNA or ZIP9-shRNA (n=5). (I and J) Immunofluorescence staining for PSD95 induced by T-BSA in HT22 cells pre-treated with nc-shRNA or ZIP9-shRNA (n=4, scale bars = 20 μm). (n.s.: not-significant; * P < 0.05; ** P < 0.01).