Research Paper Volume 11, Issue 8 pp 2281—2294

Non-genomic mechanisms mediate androgen-induced PSD95 expression

Figure 3. ZIP9/Gnα11 interactions mediate the effect of T-BSA on PSD95 expression. (A) Co-IP for the interaction between ZIP9 and Gnα11 induced by T-BSA. (B and C) Western blotting for the knockdown efficiency of Gnα11 protein in HT22 cells transfected with nc-shRNA or Gnα11-shRNA. (D and E) Duolink® proximity ligation assay for the interaction between ZIP9 and Gnα11 induced by T-BSA pre-treated with nc-shRNA, ZIP9-shRNA or Gnα11-shRNA. (F and G) Western blotting for PSD95 expression induced by T-BSA in HT22 cells pre-treated with nc-shRNA, ZIP9-shRNA or Gnα11-shRNA. (n = 5; n.s.: no-significant; * P < 0.05; ** P < 0.01).