Figure 2. Lower Foxp3 promoter methylation is detected in intratumoral T-cells from high-grade, large HCCs and correlate with %Treg in T-cells. (A) The relative methylation levels of Foxp3 promoter in intratumoral T-cells in HCC specimens with different tumor grades by MS-PCR. (B) The relative methylation levels of Foxp3 promoter in intratumoral T-cells in small versus large HCCs by MS-PCR. (C) Correlation between the methylation levels of Foxp3 promoter and the percentage of Treg cells in total intratumoral T-cells. (D) The median level of methylation level of Foxp3 promoter was used to separate methylaition-high (n=20) from methylation-low patients (n=20) to compare their overall five-year survival.*p<0.05. N=40.