Figure 4. Epigenetic alteration in intratumoral T-cells affects tumor growth. A mouse HCC cell line Hepa1-6 that expresses luciferase reporter was subcutaneously implantated into C57/BL6 mice to generate detectable tumor. From the second week after transplantation, intratumoral injection with pAAV-pCD4-DNMT1, or pAAV-pCD4-scrambled, or pAAV-pCD4-shDNMT1 was done every week till 8 weeks, when the mice were sacrificed. (A–B) The bioluminescence analysis at sacrifice, shown by representative images (A), and by quantification (B). (C–D) Measurement of dissected tumor mass, shown by representative images (C), and by quantification (D). *p<0.05. N=3.