Figure 4. HPV-mediated longer 3’UTR of RBM25 and GTF3C3 upregulates PD-1 via impacting RBPs of FUS and DGCR8. (A) In the HPV+ state, 3’UL mediates the imbalance of competing for binding of RBPs. Longer 3’UTR of RBM25 and GTF3C3 drives more RBPs to bind on them. Conversely, less RBPs will bind on PDCD1, which upregulates the expression of PDCD1. (B) PDCD1 is upregulated in the HPV+ samples. Fold change and P-value were computed through EdgeR. (C) RBPs of FUS and DGCR8 can bind to the 3’UTR of PDCD1 (CLIP-seq). CLIP-seq peaks were combined within all samples. (D) RBPs of FUS and DGCR8 can bind to the 3’UTR of RBM25. CLIP-seq peaks were combined within all samples. (E) 3’UTR level of RBM25 is significantly longer in HPV+ samples (Wilcoxon test). (F) 3’UL+ and HPV+ group shows the highest PDCD1 expression (FPKM). (G) 3’UTR length of RBM25 is positively correlated with PDCD1 expression. Spearman Rho value and P value was analyzed in HPV positive and negative samples.