Figure 2. SiRNA-mediated knockdown of LINC00857 inhibits EAC cell proliferation. (A) The LINC00857 expression level was determined by qPCR when OE19, OE33 and FLO1 cells transfected with siLINC00857. (B) WST-1 assays were used to determine the cell viability for siLINC00857-transfected OE33, FLO1 and OE19. (C) Colony-forming assays were conducted to determine the colony formation of siLINC00857-transfected OE33 and FLO1 cells. (D) The bar chart represented the count number of cloning. Values represented the mean ± s.d. from three independent experiments. *p < 0.05, **p < 0.01. (E) Flow cytometry analysis showed that the cell cycle was arrested at G1 phase after LINC00857 knockdown in OE33 cells.