Figure 1. Reduced expression of miR-3650 in HCC tissues is correlated with poor prognosis of HCC patients. (A) Relative expression of miR-3650 between HCC tissues and adjacent normal liver tissues by RT-PCR. Results were presented as Δcycle threshold (ΔCt) in tumor tissues relative to adjacent normal tissues. (B) The correlation between miR-3650 expression and tumor size: ≤ 5 cm and > 5 cm. (C) The correlation between miR-3650 expression and TNM stage: stage I and stage II-III according to the latest AJCC (American Journal of Critical Care) guide. (D) The correlation between miR-3650 expression and Barcelona Clinic Liver Cancer (BCLC) stage: stage 0+A and stage B+C. (E) and (F) Kaplan-Meier plots of overall-survival and disease-free survival in HCC patients with high (n=60) and low (n=60) levels of miR-3650. Low expression of miR-3650 was associated with reduced survival of HCC patients. (all P < 0.0001).