Research Paper Volume 11, Issue 11 pp 3574—3584

Figure 5. TGF-β1 induces cellular senescence in VSMCs through activation of ROS/NF-κB signaling. (A) Representative images of p65-NF-κB staining in control- and MFS-VSMCs. Scale bar=50 μm. (B) Western blotting and quantitative analysis of p-p65-NF-κB and p65-NF-κB levels in control- and MFS-VSMCs. (C) Western blotting and quantitative analysis of p-p65-NF-κB, p65-NF-κB, p53 and p21 levels in control-VSMCs treated with TGF-β1, TGF-β1+Mito-tempo or TGF-β1+SC-154. (D) Quantitative analysis of SA-β-gal staining in control-VSMCs treated with TGF-β1, TGF-β1+Mito-tempo or TGF-β1+SC-154. (E) Concentrations of IL-6, IL-8, TNF-α and INF-γ in medium conditioned by control-VSMCs treated with TGF-β1, TGF-β1+Mito-tempo or TGF-β1+SC-154. Data are expressed as the mean±SEM. n=3. ***p<0.001.