Research Paper Volume 11, Issue 11 pp 3601—3623

Rh type C-glycoprotein functions as a novel tumor suppressor gene by inhibiting tumorigenicity and metastasis in head and neck squamous cell carcinoma

Figure 5. Effects of RHCG on HNSCC cell proliferation, colony formation and migration. (AC) Cell growth of CAL27 and FADU cells after RHCG knockdown and JHU011 cells after RHCG overexpression as determined by CCK8 assay at different time points. (D) The proliferation of CAL27 and FADU cells after RHCG knockdown and JHU011 cells after RHCG overexpression was evaluated by EdU assay. Representative images for EdU-positive cells (red) and Hoechst-stained nuclei (blue) are shown. Scale bars represent 50 μm. Quantification of the percentage of EdU-positive cells is shown on the right. (E) Colony-forming growth assay was performed to determine the proliferation of CAL27 and FADU after RHCG knockdown and JHU011 cells after RHCG overexpression. Colonies were counted and calculated on the right. **P < 0.01 compared with the NC group. (F) Transwell migration assay of migratory capacity of CAL27 and FADU after RHCG knockdown and JHU011 cells after RHCG overexpression. Data are presented as the mean ± SD of three independent experiments on the right. *P < 0.05, **P < 0.01, compared with the NC group.