Research Paper Volume 11, Issue 11 pp 3639—3649

MORC2 promotes cell growth and metastasis in human cholangiocarcinoma and is negatively regulated by miR-186-5p

Figure 3. MORC2 knockdown impairs the migratory and invasive capacities of CCA cells. (A) The wound healing assay was used to determine the migration of MORC2 stable knockdown HuCCT1 and RBE cells. (B) Cell invasion was investigated by transwell assays in MORC2 stable knockdown HuCCT1 and RBE cells. (C) Slug, E-cadherin, N-cadherin and vimentin protein levels were detected and quantified in MORC2 stable knockdown HuCCT1 and RBE cells by western blotting. All experiments were performed in triplicate, ***P < 0.001, **P < 0.01.