Research Paper Volume 11, Issue 11 pp 3668—3678

Apigenin inhibits growth and migration of fibroblasts by suppressing FAK signaling

Figure 4. Apigenin inhibits HSF migration by suppressing FAK phosphorylation. (A) Transwell assays (without Matrigel) with HSFs overexpressing vector or FAK and treated with 20 μM apigenin,. **P < 0.05 vs. NaCl group. ## P < 0.05 vs. NaCl with FAK overexpression group. (B) Transwell assays (without Matrigel) with HSFs transfected with si-FAK or si-NC (negative control) and treated with 20 μM apigenin. **P < 0.05 vs. NaCl group. (C, D) HSFs overexpressing vector or FAK were treated with 20 μM apigenin, after which western blot and real-time PCR assays were performed to detect levels of MMP2 expression. **P < 0.05 vs. NaCl group. ## P < 0.05 vs. NaCl with FAK overexpression group. (E, F) HSFs transfected with si-FAK or si-NC (negative control) were treated with 20 μM apigenin, after which western blot and real-time PCR assays were performed to detect levels of MMP2expression. **P < 0.05 vs. NaCl group.