Research Paper Volume 11, Issue 11 pp 3731—3749

Nectandrin B-mediated activation of the AMPK pathway prevents cellular senescence in human diploid fibroblasts by reducing intracellular ROS levels

Figure 1. NecB treatment increased the cell viability of young and old HDFs. (A) Young (PD of 20) and near-senescent (PD of 72, old) HDFs were treated with vehicle (PEG) or various concentrations of NecB for 2 days and the cell viability was assessed by MTT assay. (B) Young and old HDFs were treated with 10 and 20 μg/mL NecB for 2−4 days and the cell viability was assessed by MTT assay. *P<0.05, **P<0.01, compared with vehicle-treated control.