Research Paper Volume 11, Issue 11 pp 3768—3784

Myocyte enhancer factor 2A delays vascular endothelial cell senescence by activating the PI3K/p-Akt/SIRT1 pathway

Figure 2. The influence of changes in expression of MEF2A on downstream gene expression. (A) Changes in downstream gene mRNA levels after transfection of si-MEF2A in HUVEC; (B) Changes in downstream gene protein levels after transfection of si-MEF2A in HUVEC; (C) Effect of transfection of MEF2A overexpression plasmid or transfection of MEF2A overexpression plasmid plus PI3K inhibitor on the mRNA level of downstream gene in HUVEC; (D) Influence of transfection of MEF2A overexpression plasmid or transfection of MEF2A overexpression plasmid plus PI3K inhibitors on downstream gene protein levels in HUVEC. (E) Impact of transfection of MEF2A overexpression plasmid or transfection of MEF2A overexpression plasmid plus PI3K inhibitor on HUVEC phenotype. The mRNA level, protein level and senescence rate were expressed as the mean fold changes relative to the control group, and the error bars represent the standard error of the fold changes in 3 independent experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, no significance.