Research Paper Volume 11, Issue 11 pp 3768—3784

Myocyte enhancer factor 2A delays vascular endothelial cell senescence by activating the PI3K/p-Akt/SIRT1 pathway

Figure 3. The role of MEF2A in hydrogen peroxide-induced cell senescence. (A) Effects of different concentrations of H2O2 on the viability of HUVEC cells at different time. (B) Impact of treatment with 100 μM H2O2 on the senescent phenotype of HUVEC for 1 hour. (C) Influence of treatment with 100 μM H2O2 for 1 hour on gene protein levels in HUVEC. (D) Changes in mRNA levels of genes of interest in each group. (E) Changes in protein levels of genes of interest in each group. (F) Changes in cell viability of each group. (G) Changes in the cellular senescence phenotype of each group. The cell viability, senescence rate, protein level and mRNA level were expressed as the mean fold changes relative to the control group, and the error bars represent the standard error of the fold changes in 3 independent experiments. Blank: HUVEC transfected without plasmids; pc3-gab: HUVEC transfected with empty vector (pc3-gab); pc3-gab-MEF2A-2: HUVEC transfected with MEF2A overexpression plasmid. *, P < 0.05; **, P < 0.01; ***, P < 0.001.