Research Paper Volume 11, Issue 11 pp 3795—3810

Kainic acid Induces production and aggregation of amyloid β-protein and memory deficits by activating inflammasomes in NLRP3- and NF-κB-stimulated pathways

Figure 4. Inflammasome mediates the KA-induced phosphorylation of NF-κB and the expression of NLRP3, IL-1β, and BDNF. (A and B) Phosphorylation levels of NF-κB and expression levels of NLRP3, IL-1β, and BDNF in KA and/or Bay11-7082 in the brains of APP23 mice by Western blotting. The KA group was given i.p. injections of 10 mg/kg KA. The Bay11-7082+KA group mice were given i.p. injections of 1 or 2 mg/kg Bay11-7082 for 48 h (N=6). (C and D) The expression levels of NLRP3, IL-1 β, and BDNF in KA and/or JSH-23 in the brain of APP23 mice by Western blotting. The KA group was given i.p. injections of 10 mg/kg KA. The JSH-23+KA group mice were given i.p. injections of 10 or 20 mg/kg JSH-23 for 48 h (N=6). The optical density of bands in Western blotting was analyzed by ImageJ software *P<0.05; **P<0.01; ***P<0.001 vs the control group; #P<0.05; ##P<0.01; ###P<0.001 vs the KA-only group.