Research Paper Volume 11, Issue 11 pp 3811—3823

Long non-coding RNA AGAP2-AS1 exerts oncogenic properties in glioblastoma by epigenetically silencing TFPI2 through EZH2 and LSD1

Figure 4. TFPI2 up-regulation represses proliferation and invasion, and enhanced apoptosis in GBM cells. (A and B) TFPI2 mRNA and protein levels were evaluated in U87/MG and U251/MG cells after transfection with empty vector or pcDNA-TFPI2. (C and D) CCK-8 and EdU staining assays were performed to examine the effects of TFPI2 overexpression on U87/MG and U251/MG cell proliferation. (E) Colony forming assay was conducted to detect the cloning ability in Vector- or pcDNA-TFPI2-transfected U87/MG and U251/MG cells. (F) Transwell assay of invasive ability in U87/MG and U251/MG cells following TFPI2 up-regulation. (G) Flow cytometry analysis of apoptotic rate in U87/MG and U251/MG cells introduced with empty vector or pcDNA-TFPI2. *P < 0.05, **P < 0.01.