Research Paper Volume 11, Issue 12 pp 3993—4010

Intermediate metabolites of the pyrimidine metabolism pathway extend the lifespan of C. elegans through regulating reproductive signals

Figure 4. The lifespan extension induced by thymine depends on reproductive signals. (AD) Lifespan analysis of (A) glp-1 (e2144), (B) daf-2 (e1370), (C) sir-2.1 (ok434), and (D) eat-2 (ad1116) animals treated with 2 mM thymine (red) and vehicle (black), on heat-inactivated E. coli OP50 (log-rank test). Lifespan values of replicated experiments are summarized in Supplementary Table 1. (E) The number of daily progeny and the total number of progeny of wild-type N2 worms treated with 2 mM thymine or vehicle. (F, G) DAPI-stained image of worm gonads. (H) Quantification of germline stem cells in wild-type N2 animals treated with 2 mM thymine or vehicle; n.s. not significant (Student’s t test). (I) Quantification of ORO staining. Mean ± SD.; n ≥ 30 per condition; P value was calculated using Student’s test; no significance is abbreviated as n.s. *p < 0.05, **p < 0.01, ***p < 0.001.