Figure 3. NOR MSCs were cultured at the different concentration (1,10,100,500ng/ml) of HMGB1 for 48 h. (A, B) The expressions of TLR4, p-IRAK1, p-p65 and IκBa in MSCs were determined by western blot analysis. GAPDH was used as the internal control. (C, D) BM-MSCs were fixed and stained with SA-β-gal. (E)The distribution of F-actin was disordered after treatment with exogenous HMGB1 by Immunofluorescence. (F, G) Cell viability was assessed by flow cytometry analysis. (Bar represents mean ± SD,*P < 0.05 compared with the normal group, #P < 0.05 compared with the normal group, &P < 0.05 compared with the normal group) (NOR=normal group, SLE=systemic lupus erythematosus patients group).