Research Paper Volume 11, Issue 13 pp 4579—4586

Ketones improves Apolipoprotein E4-related memory deficiency via sirtuin 3

Figure 3. Ketones upregulate Sirt3, PSD95 and Synaptophysin in ApoE 4 mice. ApoE4 and ApoE3 mice were treated with either ketones or normal saline for three months. Fresh hippocampal and cortical tissues were collected and homogenized for western blot. (AD) Proteins levels were measured in the hippocampus: (A) Representative western blot bands for Sirt3, PSD95, Synaptophysin, and Actin were shown. (B) Sirt3, (C) PSD95 and (D) Synaptophysin levels were normalized with Actin. (EH) Proteins levels were measured in the cortex: (E) Representative western blot bands for Sirt3, PSD95, Synaptophysin and Actin were shown. (F) Sirt3, (G) PSD95 and (H) Synaptophysin levels were normalized with Actin. N=8 per group, # p<0.05, ApoE3-NS vs. ApoE4-NS; * p<0.05, ApoE4-NS vs. ApoE4-Ket.