Figure 1. Effects of knockdown of MIR22HG on EAC cells viability. (A) relative expression of MIR22HG in OE33, FLO-1 and OE19 cell lines. (B) The MIR22HG expression level indicating the knockdown efficiency of siRNA determined by qRT-PCR in 3 EAC cells transfected with siMIR22HG. (C) WST-1 assays were used to determine the cell viability after MIR22HG knockdown with siRNA in OE33, FLO-1 and OE19 cells. (D), Colony formation in OE33 and FLO1 cells after MIR22HG knockdown. (E) Bar chart counting the number of colonies from Figure 1D. Values represented the mean ± s.d. from three independent experiments. **P < 0.01.