Figure 3. Proteins and mRNAs regulated by knockdown of MIR22HG. (A) Protein levels of t-STAT3, p-STAT3 and p-FAK were regulated by MIR22HG siRNA in OE33 and FLO cells, and c-Myc was changed in FLO1 cells by Western blotting. PARP cleavage was also induced by MIR22HG siRNA in OE33 and FLO1 cells. GAPDH was used as a protein loading control. (B) qRT-PCR showing the mRNA expressions of STAT3, c-MYC and FAK in OE33 and FLO1 cells. GAPDH was used as control. (C) MET, EGFR, AKT and ERK1/2 proteins were not changed after MIR22HG siRNA treatment at 72 hours.