Research Paper Volume 11, Issue 13 pp 4706—4719

Tea polyphenol protects against cisplatin-induced meiotic defects in porcine oocytes

Figure 3. Effects of TP on the acetylation level of α-tubulin in DDP-exposed porcine oocytes. (A) Representative images of acetylated α-tubulin in control, DDP-exposed and TP-supplemented oocytes. Scale bar, 5 μm. (B) Quantitative analysis of the fluorescence intensity of acetylated α-tubulin in control, DDP-exposed and TP-supplemented oocytes. (C) The acetylation levels of α-tubulin were detected by Immunoblotting in control, DDP-exposed and TP-supplemented oocytes. The blots were probed with anti-acetyl-α-tubulin and anti-Gapdh antibodies, respectively. Data were presented as mean percentage (mean ± SEM) of at least three independent experiments. **P < 0.01, ***P < 0.001.