Research Paper Volume 11, Issue 14 pp 4943—4969

D-ribose is elevated in T1DM patients and can be involved in the onset of encephalopathy

Figure 2. Effect of benfotiamine (BTMP) on the levels of D-ribose, D-glucose and TKT in T1DM rats. Conditions for the preparation of T1DM rats are shown in Figure 1. Male rats (6-8 weeks) were divided into four groups as follows: T1DM rats were gavaged with benfotiamine (BTMP, 300 mg/kg bw, once daily) dissolved in carboxymethylcellulose (CMC) (90) (T1DM+BTMP, n=20); T1DM rats were gavaged with CMC (T1DM, n=20); normal SD rats were gavaged with CMC (Control, n=10) and BTMP (n=10) as negative and positive controls, respectively. The expression levels of transketolase (TKT) in the brain (panel A) and liver (panel B) were measured with ELISA kits. After 10 weeks of domestication, D-ribose levels in the serum (panel C) and brain (panel D) of rats were measured, and D-glucose levels were measured in the brain (panel F). Fasting blood glucose (FBG) was measured every other week (panel E). “*” compared to the control group. “#” represents the difference between the T1DM and T1DM+BTMP groups. All values are expressed as the mean ± S.E.M. *, P < 0.05; ***, P < 0.001; #, P < 0.05; ##, P < 0.01; ###, P < 0.001.