Figure 4. Nissl staining of hippocampal neurons of rats treated with BTMP. Animal groups and treatment were as described in Figure 2 except that hippocampal slices were prepared and stained with cresyl violet (panel A). Numbers of necrotic neurons were counted under a microscope as described in the Materials and Methods (panel B). The number of those groups are control (n=10), BTMP (n=9), T1DM (n=10) and T1DM+BTMP (n=10). All values are expressed as the mean ± S.E.M. “*” compared to the control group. “#” represents the difference between the T1DM and T1DM+BTMP groups. All values are expressed as the mean ± S.E.M. **, P < 0.01; #, P < 0.05; ##, P < 0.01.