Research Paper Volume 11, Issue 14 pp 5140—5157

Subunit contribution to NMDA receptor hypofunction and redox sensitivity of hippocampal synaptic transmission during aging

Figure 6. Decrease in NMDAR synaptic responses in young animals, under oxidizing conditions, depends on CaMKII activity. (A) Time course of normalized NMDAR-fEPSP slope following application of DTNB (0.5 mM, arrow) in the young animals. Each point represents the mean (±SEM), normalized to the baseline (dashed line), for slices in the control condition (open circles) or following pre-incubation with the CaMKII inhibitor, KN-62 (10 µM, filled circles). DTNB reduced NMDAR synaptic response. Pre-incubation with KN-62 blocked the decrease in the NMDAR response associated with DTNB application. (B) Quantification of the mean percent change in the NMDAR-fEPSP slope during the last 5 min of recording in the presence of vehicle control (open, n = 7/7 slices/animals) and KN-62 (, n = 7/7 slices/animals). Pound sign indicates a significant difference between the two groups. The waveforms represent examples of NMDAR-fEPSPs recorded during baseline (1) and 60 min following application of DTNB (2) in the control condition (left) and following pre-incubation in KN-62 (right).