Research Paper Volume 11, Issue 15 pp 5351—5367

Long intergenic non-protein coding RNA 511 promotes the progression of osteosarcoma cells through sponging microRNA 618 to upregulate the expression of maelstrom

Figure 4. LINC00511 promotes the tumorigenesis of OS in nude mice. (A) Xenografts formed by HOS cells with normal (upper) or downregulated (bottom) expression of LINC00511. Tumor tissues were harvested after 3 weeks of implantation (Scale bar, 0.5 cm). (B) Expression level of LINC00511 in the xenografts tissues. *P<0.05 compared with NC group. (C–D) SiRNA-induced downregulation of LINC00511 inhibited the growth of xenografts in nude mice. The volumes of xenografts were measured every 3 days, and their weights were measured after harvesting. (E) Xenografts formed by HOS cells with normal (upper) or downregulated (bottom) expression of LINC00511. Tumor tissues were harvested after 3 weeks of implantation (Scale bar, 0.5 cm). Lenti-sh-LINC00511 cells were transfected with lentivirus-mediated shRNA targeting LINC00511. Lenti-sh-ctrl means cells were transfected with shRNA lentiviral particles with nontargeting scrambled shRNA sequences. (F) The LINC00511 expression level in lenti-sh-LINC00511 group was markedly decreased compared to that in the lenti-sh-ctrl group. (GH) ShRNA-induced downregulation of LINC00511 inhibited the growth of xenografts in nude mice. The volumes of xenografts were measured every 3 days, and their weights were measured after harvesting. *P<0.05 compared to the lenti-sh-ctrl group.