Immune system development and age-dependent maintenance in Klotho-hypomorphic mice

Figure 2.Immune cells in the bone marrow have a similar composition in C57BL/6 and B6-kl/kl mice. (A) Total bone marrow (BM) cells in C57BL/6 and B6-kl/kl at 4-19 weeks (C57BL/6 n=15 and B6-kl/kl n=18) of age or 20+ weeks (C57BL/6 n=4 and B6-kl/kl n=18) from pooled male and female mice. Statistical significance determined by multiple t tests: **** p ≤ 0.0001. (B) Representative flow cytometry plot and (C) frequency of MPP and (D) HSC of the LSK (Lin^{-}cKit^{+}Sca1^{+}) cells. C57BL/6 n=15, 4-19 weeks; n=4, 20+ weeks. B6-kl/kl n=18, 4-19 weeks; n=18, 20+ weeks. Statistical significance determined by 2way ANOVA and Tukey’s multiple comparison test: *** p≤ 0.001 and **** p≤ 0.0001. (E) Representative flow cytometry plot of CLP (Lin^{-}CD127^{+}AA4.1^{+}Sca1^{low}), and (F) frequency of CLP in of Lin^{-}CD127^{+} cells. C57BL/6 n=15, 4-19 weeks; n=4, 20+ weeks. B6-kl/kl n=18, 4-19 weeks; n=18, 20+ weeks. Statistical significance determined by 2way ANOVA and Tukey’s multiple comparison test: **** p ≤ 0.0001. (G) Representative flow cytometry plot of Myeloid cells (CD11b^{+}CD11c^{-}) and (H) frequency of CD11b^{+}CD11c^{-} cells of B220^{-}CD19^{-} cells. C57BL/6 n=10, 4-19 weeks; n=4, 20+ weeks. B6-kl/kl n=15, 4-19 weeks; n=15, 20+ weeks. (I) Representative flow cytometry plot for Pre B cells (B220^{low}CD43^{-}), Pro B cells (B220^{+}CD43^{+}) and new B cells (B220^{+}IgM^{+}) and (J) frequency of B cell subsets from BM. C57BL/6 n=15, 4-19 weeks; n=4, 20+ weeks. B6-kl/kl n=18, 4-19 weeks; n=16, 20+ weeks. Statistical significance determined by 2way ANOVA and Tukey’s multiple comparison test: **** p ≤ 0.0001.