Research Paper Volume 11, Issue 14 pp 5246—5257

Immune system development and age-dependent maintenance in Klotho-hypomorphic mice

Figure 2. Immune cells in the bone marrow have a similar composition in C57BL/6 and B6-kl/kl mice. (A) Total bone marrow (BM) cells in C57BL/6 and B6-kl/kl at 4-19 weeks (C57BL/6 n=15 and B6-kl/kl n=18) of age or 20+ weeks (C57BL/6 n=4 and B6-kl/kl n=18) from pooled male and female mice. Statistical significance determined by multiple t tests: **** p ≤ 0.0001. (B) Representative flow cytometry plot and (C) frequency of MPP and (D) HSC of the LSK (Lin-cKit+Sca1+) cells. C57BL/6 n=15, 4-19 weeks; n=4, 20+ weeks. B6-kl/kl n=18, 4-19 weeks; n=18, 20+ weeks. Statistical significance determined by 2way ANOVA and Tukey’s multiple comparison test: *** p≤ 0.001 and **** p≤ 0.0001. (E) Representative flow cytometry plot of CLP (Lin-CD127+AA4.1+Sca1low), and (F) frequency of CLP in of Lin-CD127+ cells. C57BL/6 n=15, 4-19 weeks; n=4, 20+ weeks. B6-kl/kl n=18, 4-19 weeks; n=18, 20+ weeks. Statistical significance determined by 2way ANOVA and Tukey’s multiple comparison test: **** p ≤ 0.0001. (G) Representative flow cytometry plot of Myeloid cells (CD11b+CD11c-) and (H) frequency of CD11b+CD11c- cells of B220-CD19- cells. C57BL/6 n=10, 4-19 weeks; n=4, 20+ weeks. B6-kl/kl n=15, 4-19 weeks; n=15, 20+ weeks. (I) Representative flow cytometry plot for Pre B cells (B220lowCD43-), Pro B cells (B220+CD43+) and new B cells (B220+IgM+) and (J) frequency of B cell subsets from BM. C57BL/6 n=15, 4-19 weeks; n=4, 20+ weeks. B6-kl/kl n=18, 4-19 weeks; n=16, 20+ weeks. Statistical significance determined by 2way ANOVA and Tukey’s multiple comparison test: **** p ≤ 0.0001.