Research Paper Volume 11, Issue 15 pp 5445—5462

Impact of C-reactive protein on osteo-/chondrogenic transdifferentiation and calcification of vascular smooth muscle cells

Figure 7. Silencing of FCGR2A inhibits CRP-induced osteogenic signaling and calcification of HAoSMCs. (A-E) Scatter dot plots and arithmetic means ± SEM (n=6; arbitrary units, a.u.) of FCGR2A (A), NOX4 (B), CYBA (C), CBFA1 (D) and ALPL (E) relative mRNA expression in HAoSMCs silenced with negative control siRNA (Neg.si) or FCGR2A siRNA (FR2Asi) and treated with control or 10 µg/ml recombinant human CRP. (F) Scatter dot plots and arithmetic means ± SEM (n=6; µg/mg protein) of calcium content in HAoSMCs silenced with negative control siRNA (Neg.si) or FCGR2A siRNA (FR2Asi) and treated with control or calcification medium together with 10 µg/ml recombinant human CRP (Calc.+CRP). *(p<0.05), **(p<0.01), ***(p<0.001) significant vs. Neg.si silenced HAoSMCs; ††(p<0.01), †††(p<0.001) significant vs. Neg.si silenced and CRP/Calc.+CRP treated HAoSMCs.