Research Paper Volume 11, Issue 15 pp 5445—5462

Impact of C-reactive protein on osteo-/chondrogenic transdifferentiation and calcification of vascular smooth muscle cells

Figure 8. Vascular CRP expression is increased during pro-calcifying conditions. (A) Scatter dot plots and arithmetic means ± SEM (n=6-7; arbitrary units, a.u.) of Crp relative mRNA expression in aortic tissue of klotho-hypomorphic (kl/kl) mice and corresponding wild-type (WT) mice. (B) Scatter dot plots and arithmetic means ± SEM (n=5; a.u.) of CRP relative mRNA expression in HAoSMCs treated with control (CTR) or calcification medium (Calc.). (C) Representative original Western blots and scatter dot plots and arithmetic means ± SEM (n=9; a.u.) of normalized CRP/GAPDH protein ratio in HAoSMCs treated with control (CTR) or calcification medium (Calc.). *(p<0.05), **(p<0.01) significant vs. WT mice or control HAoSMCs, respectively.