Research Paper Volume 11, Issue 15 pp 5483—5497

CBX3/HP1γ promotes tumor proliferation and predicts poor survival in hepatocellular carcinoma

Figure 5. CBX3/HP1γ was stably overexpressed in HCC cell lines and promoted cell proliferation. (A and B) Overexpression of CBX3/HP1γ (OE) in a transfected SK-Hep-1 and SMMC-7721 cell lines compared to cells transfected with the control vector (Vec) was verified by Western blotting. (C) qPCR confirmed overexpression of CBX3/HP1γ mRNA in SK-Hep-1 and SMMC-7721 cell lines. (D and E) Cell colony formation assay results showing that CBX3/HP1γ overexpression promoted cell proliferation in SK-Hep-1 and SMMC-7721 cells (OE) compared to cells transfected with control vector (Vec). (F) CCK8 assay results showing CBX3/HP1γ overexpression accelerated cell growth rates in SK-Hep-1 and SMMC-7721 cells (OE) compared to cells transfected with control vector (Vec).