Figure 2. FEN1 promotes HCC cell migration, invasion and EMT in vitro. (A and C) Representative images of wound-healing assays and (B and D) quantification of wound closure. (E and G) Representative images of transwell chamber assays and (F and H) quantification of the number of cells which migrated or invaded through the basement membrane. Data represent mean ± SD of three independent experiments, **P < 0.01. (I) Western blot analysis of EMT markers, FEN1, MMP2 and MMP9 after silencing of FEN1. (J) Western blot analysis of EMT markers, FEN1, MMP2 and MMP9 after FEN1 overexpression.