Research Paper Volume 11, Issue 15 pp 5666—5688

Overexpressed pseudogenes, DUXAP8 and DUXAP9, promote growth of renal cell carcinoma and serve as unfavorable prognostic biomarkers

Figure 7. Pseudogenes DUXAP8 and DUXAP9 promote tumor growth via suppression of miR-29c-3p-COL1A1/COL1A2 axis in renal cell carcinoma. (A) The expression of pseudogene DUXAP8 in renal cell carcinoma samples and normal controls; (B) the expression of pseudogene DUXAP9 in renal cell carcinoma samples and normal controls; (C) the expression of miR-29c-3p in renal cell carcinoma samples and normal controls; (D) the expression of COL1A1 in renal cell carcinoma samples and normal controls; (E) the expression of COL1A2 in renal cell carcinoma samples and normal controls; (F) miR-29c-3p suppressed Renilla luciferase activity of the reporters containing the wild-type but not mutant DUXAP8 in ACHN cell line; (G) miR-29c-3p suppressed Renilla luciferase activity of the reporters containing the wild-type but not mutant DUXAP9 in A498 cell line; (H) miR-29c-3p suppressed Renilla luciferase activity of the reporters containing the wild-type but not mutant COL1A1 in ACHN cell line; (I) miR-29c-3p suppressed Renilla luciferase activity of the reporters containing the wild-type but not mutant COL1A2 in A498 cell line; (J) the knockdown effect of si-DUXAP8 and si-DUXAP9 in ACHN and A498 cell lines; (K) the overexpression effect of miR-29c-3p mimic in ACHN and A498 cell lines; (L) knockdown of pseudogene DUXAP8/DUXAP9 or/and overexpression of miR-29c-3p inhibited cell growth in vitro; (M) knockdown of pseudogene DUXAP8/DUXAP9 or/and overexpression of miR-29c-3p suppressed the colony formation of cell lines in renal cell carcinoma. “*” represents P-value less than 0.05. Abbreviations: MUT, mutant; WT, wild type.