Figure 2. PTN is a direct target of miR-627-3p in osteosarcoma. (A) miRDB predicted that miR-627-3p could be specifically combined with PTN. (B) Real time PCR analysis of PTN mRNA expression in osteosarcoma tissues and adjacent tissues. Data are the mean ± SEM. ** P< 0.05 vs. adjacent tissues group. (C) Relationship between PTN and survival period among osteosarcoma patients. (D) Real time PCR analysis of PTN expression in hFOB 1.19, HOS, U2OS and MG63 cells. ** P< 0.05 vs. hFOB 1.19 cells. (E) Correlation between expression of miR-627-3p and PTN in osteosarcoma. (F) Luciferase reporter assays testing the interaction between miR-627-3p and the PTN 3′-UTR. Data are the mean ± SEM. ** P< 0.05 vs. control group. (G–J) Western blot and real time PCR analyses showing expression of PTN after miR-627-3p overexpression or inhibition. Data are shown as the mean ± SEM. ** P< 0.05 vs. control group.