Research Paper Volume 11, Issue 15 pp 5744—5756

miR-627-3p inhibits osteosarcoma cell proliferation and metastasis by targeting PTN

Figure 3. MiR-627-3p inhibits osteosarcoma cell proliferation. (A, B) MTT assays showing cell proliferation after transfection with miR-627-3p mimic/inhibitor in HOS, MG63 and MG63/dox cells. Data are the mean ± SEM. ** P< 0.05 vs. control group. (C, D) Colony formation assays showing HOS and MG63 cell proliferation after transfection with miR-627-3p mimic/inhibitor. Data are the mean ± SEM. ** P< 0.05 vs. control group. (E, F) AV-PI assay showing cell apoptosis among HOS cells transfected with miR-627-3p mimic/inhibitor. (GJ) Western blot and real-time PCR analyses of Cyclin D1 expression in osteosarcoma cells transfected with miR-627-3p mimic/inhibitor. Data are the mean ± SEM. ** P< 0.05.