Expression of Concern
This article is currently under investigation. We strongly recommend that this article is not cited until the investigation is completed.
Research Paper Volume 11, Issue 15 pp 5744—5756

miR-627-3p inhibits osteosarcoma cell proliferation and metastasis by targeting PTN

class="figure-viewer-img"

Figure 5. MiR-627-3p suppresses HOS cell proliferation and migration by targeting PTN. (A) MTT assays showing the effects of miR-627-3p inhibitor and PTN siRNA on cell proliferation. Data are the mean ± SEM. ** P< 0.05, control vs miR-627-3p inhibitor/si-PTN; ^^P< 0.05, miR-627-3p inhibitor vs si-PTN. (B) Transwell assays showing the effects of miR-627-3p inhibitor and PTN siRNA on cell metastasis. Cells were counted, and results represent the mean ± SD of three experiments. ** P< 0.05, control vs miR-627-3p inhibitor/si-PTN; ^^P< 0.05 miR-627-3p inhibitor vs si-PTN. (C, D) Western blot and real-time PCR analysis of the effects of miR-627-3p inhibitor and PTN siRNA on expression of PTN, Cyclin D1 and MMP2. Data are the mean ± SEM. ** P< 0.05, control vs miR-627-3p inhibitor/si-PTN; P< 0.05 miR-627-3p inhibitor vs si-PTN. (E) MTT assays showing the effects of miR-627-3p and PTN on cell proliferation. Data are the mean ± SEM. ** P< 0.05, control vs miR-627-3p/PTN; ^^P< 0.05 miR-627-3p vs PTN. (F) Transwell assays showing the effects of miR-627-3p and PTN on cell metastasis. Shown are counts of migrated cells. Results represent the mean ± SD of three experiments. **P< 0.05, control vs miR-627-3p/PTN; ^^P< 0.05, miR-627-3p vs PTN. (G, H) Western blot and real-time PCR analysis of the effects of miR-627-3p and PTN on PTN, Cyclin D1 and MMP2 expression. Data are the mean ± SEM. ** P< 0.05, control vs miR-627-3p/PTN; ^^P< 0.05 miR-627-3p vs PTN.