Research Paper Volume 11, Issue 17 pp 6674—6690

RPN2 promotes metastasis of hepatocellular carcinoma cell and inhibits autophagy via STAT3 and NF-κB pathways

Figure 2. RPN2 overexpression promotes proliferation of Huh-7 and HepG2 cells. The cell lines were transfected with AD-RPN2 and AD-NC (control). Western blotting (A, B) and qPCR (C, D) were conducted to confirm RPN2 overexpression in both the cell lines. (E, F) Multiplication of Huh-7 and HepG2 cells was measured at time points of 12, 24, 36, 48, 60, and 72 h after transfection by the MTT assay. (G, H) Soft agar colony formation assay of the Huh-7 and HepG2 cells expressing RPN2 and controls. (I) The NHC were transfected with AD-RPN2 and AD-NC (control). WB was conducted to confirm RPN2 overexpression in NHC. (J) Multiplication of NHC was measured at time points of 12, 24, 36, 48, 60, and 72 h after transfection by the MTT assay. The band of target protein was normalized to the density of action. The quantification was performed independently in a single band. The experiments were performed three times. Data are recorded as mean ± SD. **P <0.01 vs control group.