Research Paper Volume 11, Issue 17 pp 6714—6733

MeCP2 inhibits cell functionality through FoxO3a and autophagy in endothelial progenitor cells

Figure 8. MeCP2 inhibits FoxO3a transcription by promoting FoxO3a promoter methylation and enhancing H3K9 dimethylation. (A) Genome methylation was detected after MeCP2 overexpression. (B) DNA methylation was detected by BSP after MeCP2 overexpression. (C) ChIP of MeCP2 binding to the FoxO3a promoter after MeCP2 overexpression. (D) ChIP demonstrated H3K9me2 enrichment on the FoxO3a promoter after MeCP2 overexpression. (E) Proposed mechanism. *P < 0.05, **P < 0.01, and ***P < 0.001 vs. control.