Figure 9. MiR-330-3p promoted NSCLC cells migration and invasion via TGF-β-induced EMT process. (A) Western blotting analysis of EMT related protein levels in the indicated cells. miR-330-3p could increase mesenchymal phenotypic biomarkers expression and decrease E-cadherin expression. (B) Immunofluorescence staining detected the Vimentin and E-cadherin expression in A549 and HCC827 cells. (C, D) Western blotting analysis of EMT associated protein expression in A549 and HCC827 cells treated with recombinant TGF-β1 (C) and the TGF-β1 inhibitor LY2109761 (D), respectively. (E) Co-IP and Western blotting indicating the endogenous interaction between GRIA3 and TGF-β1 protein in HCC827 cells. (F) Western blotting analysis evaluated TGF-β1 expression in A549 and HCC827 cells overexpressing GRIA3.