Research Paper Volume 11, Issue 18 pp 7357—7385

miR-375-3p suppresses tumorigenesis and partially reverses chemoresistance by targeting YAP1 and SP1 in colorectal cancer cells

Figure 7. Expression of SP1, YAP1 and its downstream proteins, apoptosis-related proteins were regulated by miR-375-3p. (A) Transfection of miR-375-3p mimics into HCT116/FU cells with or not with 5FU treatment inhibited the expression of Hippo-YAP1 pathway-associated downstream proteins. Expression of the indicated proteins was detected by western blot. (B) Transfection of miR-375-3p inhibitors into HCT116 cells promoted the expression of Hippo-YAP1 pathway-associated downstream proteins. Expression of the indicated proteins was detected by western blot. (C) The inhibited effects of miR-375-3p on Hippo-YAP1 pathway-related downstream proteins were abolished by transfection of YAP1 overexpressing plasmid in HCT116/FU cells. (D) The promoted effects of miR-375-3p inhibitors on Hippo-YAP1 pathway-related downstream proteins were reversed by transfection of siYAP1 in HCT116 cells. (E, F) The apoptosis-related proteins expression levels were detected in HCT116/FU cells transfected with miR-375-3p (left) or siYAP1/siSP1 (right). MiR-375-3p and siYAP1 and siSP1 promotes apoptosis in protein level. (G, H) The apoptosis-related proteins expression levels were detected in HCT116 cells transfected with miR-375-3p inhibitors (left) or YAP1/SP1 overexpressing plasmids (right). MiR-375-3p inhibitors and YAP1 or SP1 inhibits apoptosis in protein level.