Research Paper Volume 11, Issue 18 pp 7386—7401

lncRNA DLEU2 modulates cell proliferation and invasion of non-small cell lung cancer by regulating miR-30c-5p/SOX9 axis

Figure 3. miR-30c-5p was the target of lncRNA DLEU2. (AB) The expression of miR-30c-5p in NSCLC tissues and cell lines were measured by qRT-PCR, ##p<0.01, compared with adjacent tissues; **p<0.01, compared with BEAS-2B cells; (C) Spearman’s correlation analysis was used to evaluate the expression relationship between lncRNA DLEU2 and miR-30c-5p; (D) The expression of miR-30c-5p in A549 and LLC cells were transferred with si-DLEU2 and pcDNA-DLEU2 by qRT-PCR; (E) The bioinformatics analysis result showed that lncRNA DLEU2 had a binding site with miR-30c-5p; (F) Dual-luciferase reporter gene assay was used to confirm the target relationship between lncRNA DLEU2 and miR-30c-5p. ΔΔp<0.01, ΔΔΔp<0.001, compared with the NC group.